Fig. 2. Effects of miR-9-5p on HSC activation in vitro and in vivo. LX-2 cells were treated with TGF-β1 for 24 h and then transfected with miR-9-5p mimics for 24 h. (A) miR-9-5p expression. (B) Cell proliferation detected by MTT. (C) α-SMA mRNA. (D) Col1A1 mRNA. (E) α-SMA protein. (F) Type I collagen. (G and H) Collagen deposits were analyzed by Sirius Red staining. Scale bars, 100 µm. (I) Hydroxyproline expression. (J) Cell proliferation detected by EdU. Each value is the mean ± SD of three experiments. *P<0.05 compared with the control and #P<0.05 compared with TGF-β1 or CCl4 group.